Phospho-slp-76 ser376

WebMay 2024 - Sep 20241 year 5 months. 1250 S Collegeville Rd, Collegeville, PA 19426. • Demonstrated the in vivo efficacy of various GSK’s proprietary small molecules for their … Webtion of SLP-76 at Ser376, but does not affect the level of total SLP-76 protein. The relationship between lysate protein concentration from untreated and H 2 O -treated Jurkat cells and the absorbance at 450 nm using the FastScan™ Phospho-SLP-76 (Ser376) ELISA Kit #30794 is shown in the upper figure. The corresponding western blots using

Purified anti-SLP76 Phospho (Ser376) Antibody - BioLegend

WebPhospho-SLP-76 (Ser376) Sandwich ELISA kit #78222. A lower signal of phospho-SLP-76 protein is seen in the untreated lysate. However, similar levels of total SLP-76 protein from both untreated and treated lysates are shown by Western analysis. The absorbance readings at 450 nm are shown in the top figure, WebLymphocyte cytosolic protein (SLP-76) is required for T-cell activation and has been implicated in a number of signaling pathways. SLP76 is phosphorylated at Ser376 by HPK1, and this phosphorylation leads to a decrease in T-cell receptor signaling. o Description of the LANCE Ultra Assay the park menu burlington nc https://aspenqld.com

PE anti-SLP76 Phospho (Ser376) - sonybiotechnology.com

WebListed are ELISA Kits for the detection of SLP76, an alias name of lymphocyte cytosolic protein 2. The human protein, encoded by the gene LCP2, is 533 amino acid residues long and has a mass of 60,188 daltons. This protein is reported to have a cytoplasmic cellular localization. This target may also be known as: 76 kDa tyrosine phosphoprotein ... Webnm using the FastScan™ Phospho-SLP-76 (Ser376) ELISA Kit (Human Preferred) #25424 is shown in the upper figure. The cor-responding western blots using phospho-SLP-76 (Ser376) antibody (left panel) and SLP-76 antibody (right panel) are shown in the lower figure. After serum starvation, Jurkat cells were either left untreated or treated with 11 ... WebJan 20, 2024 · We report here that SLP76-S376A-expressing mice exhibit normal thymocyte development and no detectable phenotypic alterations in mature T cell subsets or other lymphoid and myeloid cell lineages. Biochemical analyses revealed that mutant T cells were hypersensitive to TCR stimulation. shuttle tours of america

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Category:Purified anti-SLP-76 Antibody anti-SLP-76 - H76

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Phospho-slp-76 ser376

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WebNo-wash kit for detection and quantitation of phospho-SLP-76 in cells using AlphaLISA SureFire technology. We are now two standalone organizations Life Sciences & … WebSLP-76 was originally identified as a substrate of the ZAP-70 protein tyrosine kinase following T cell receptor (TCR) ligation in the leukemic T cell line Jurkat. The SLP-76 locus …

Phospho-slp-76 ser376

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WebSLP76 is a cytosolic adapter protein that functions as a substrate in the T cell receptor (TCR) activation pathway. Upon TCR engagement, SLP76 is phosphorylated on multiple residues, resulting in the protein being … WebSLP-76 phosphorylated at Ser376 and another that recognizes an invariant epitope of SLP-76. ... Eu-Ab1 and FR-Ab2 (5 µL) for detection of phospho-SLP-76 (S376). • The plate was incubated at RT for . 18 hours. and the TR-FRET signal . was recorded at 665 and 615 nm (EnVision®; lamp excitation).

Webendogenous SLP-76 at Ser376, in cellular lysates. The assay is an ideal system for the screening of both modulators of receptor activation (e.g. agonists and antagonists) as …

WebSLP-76 can be phosphorylated on multiple tyrosine residues by the upstream kinases ZAP-70 and Lck. SLP-76 phosphorylation plays an important role in T cell-mediated IL-2 production by allowing phosphorylated Vav to bind; this complex (SLP-76/Vav) stimulates NF-AT and IL-2 gene activation after TCR engagement. WebThis assay kit contains antibodies which recognize the phospho-Ser376 epitope, and a distal epitope, on SLP-76. The protein detected by this kit corresponds to UniProt ID Q13094. …

WebAs Phospho SLP76 (Ser396) is a readout of T cell activation, it was used to monitor the inhibitory effect of PD-L1 recombinant protein on T cell activation. This assay was performed using the two-plate assay protocol. 400 000 Jurkat cells were plated in 25 µL of complete RPMI culture medium with 10% FBS.

WebThis assay kit contains antibodies which recognize the phospho-Ser376 epitope, and a distal epitope, on SLP-76. The protein detected by this kit corresponds to UniProt ID Q13094. uSLP-76 is also known as lymphocyte cytosolic protein 2. These antibodies recognize SLP-76 of human origin. Other species should be tested on a case-by-case basis. shuttle touchscreen pc with windowsWebJan 1, 2024 · Product Description. Phosphor- and total SLP-76 assays for monitoring T cell receptor signaling Cisbio cell-based homogeneous htrf phospho-slp-76 (Ser376) … the park menu rapid cityWebMouse phospho-SLP-76 (Ser376) control lysate HTRF® This lysate is a component of the mouse phospho-SLP-76 (Ser376) kit. It may be used as a residue specific positive control for mouse SLP-76 phosphorylation. See more Size Cat. number selected : - Quantity Request for quotation Prices may differ between countries. the park menu austinWebThe Mouse Total SLP-76 cellular assay kit is designed to monitor the expression level of SLP-76, whether phosphorylated or unphosphorylated. It is compatible with our Mouse phospho-SLP-76 (Ser376) kit, and enables the analysis of phosphorylated and total proteins from a single sample for better readouts of T-lymphocyte activation. Benefits the park melbourneWebThis assay kit contains antibodies which recognize the phospho-Ser376 epitope, and a distal epitope, on SLP-76. The protein detected by this kit corresponds to GenBank Accession U20158.1. These antibodies recognize SLP-76 of human origin. Other species should be tested on a case-by-case basis. the park menu rapid city sdWebThe Phospho-SLP-76 (Tyr145) assay measures SLP-76 when phosphorylated at Ser376. Unlike Western Blot, the assay is entirely plate-based and does not require gels, electrophoresis, or transfer. The Phospho-SLP-76 (Tyr145) assay uses 2 labeled antibodies: one with a donor fluorophore, the other with an acceptor. shuttle to valley river casinoWebhuman phospho-SLP-76 (Ser376) as compared to kit #30794. Background: SH2 domain-containing leukocyte protein of 76 kDa (SLP-76) is a hematopoietic adapter protein that is … shuttle to valley of fire